Defense ministry spokesman, Gen. Zahiri Azimi, said at least 39 civilians including women and children were also killed and 88 others were injured.
Gen. Azimi further added that 176 militants were killed and 95 others were injured during the same day across the country.
According to Azimi, violence during the runoff vote was considerably high as compared to the first round of election on April 5th.
He said at least 506 threats were recorded during the runoff vote which includes 231 improvised explosive device (IED) attacks, 1 suicide attack and 273 direct fires.
Azimi said at least 8 Afghan soldiers were martyred and 44 others were injured during the first round of election, while 164 militants were killed and 82 others were injure during the same period.
Despite the threat of violence, a large number of Afghans participate in the runoff vote to elect the next president of Afghanistan.
Independent Election Commission (IEC) Chief, Ahmad Yousuf Nuristani said around seven million people including 62 percent men and 38 percent women participated in runoff vote.